Abstract
Background: Quinonemethide triterpenoids, known as celastroloids, constitute a relatively small group of biologically active compounds restricted to the Celastraceae family and, therefore, they are chemotaxonomic markers for this family. Among this particular type of metabolite, pristimerin and tingenone are considered traditional medicines in Latin America. The aim of this study was the isolation of the most abundant celastroloids from the root bark of Maytenus chiapensis, and thereafter, to develop an analytical method to identify pristimerin and tingenone in the Celastraceae species. Methods: Pristimerin and tingenone were isolated from the n-hexane-Et2O extract of the root bark of M. chiapensis through chromatographic techniques, and were used as internal standards. Application of a validated RP HPLC-PDA method was developed for the simultaneous quantification of these two metabolites in three different extracts, n-hexane-Et2O, methanol, and water, to determine the best extractor solvent. Results: Concentration values showed great variation between the solvents used for extraction, with the n-hexane–Et2O extract being the richest in pristimerin and tingenone. Conclusions: M. chiapensis is a source of two biologically active quinonemethide triterpenoids. An analytical method was developed for the qualification and quantification of these two celastroloids in the root bark extracts of M. chiapensis. The validated method reported herein could be extended and be useful in analyzing Celastraceae species and real commercial samples.
Highlights
Species of the Celastraceae family have had a long history in traditional medicine and agriculture inNorth Africa, South and Central America, and Central and East Asia [1]
quinonemethide triterpenoids (QMTs) constitute a relatively small group of biologically active compounds restricted to the Celastraceae family, commonly referred to as the bittersweet family [3] and, they are considered to be chemotaxonomic markers for this family
Taking into consideration the relevance of QMTs from a chemotaxonomic and therapeutic point of view, the aim of our study is to develop a validated analytical method to identify pristimerin and tingenone in the root barks of Celastraceae species
Summary
Species of the Celastraceae family have had a long history in traditional medicine and agriculture inNorth Africa, South and Central America, and Central and East Asia [1]. QMTs constitute a relatively small group of biologically active compounds restricted to the Celastraceae family, commonly referred to as the bittersweet family [3] and, they are considered to be chemotaxonomic markers for this family. Quinonemethide triterpenoids, known as celastroloids, constitute a relatively small group of biologically active compounds restricted to the Celastraceae family and, they are chemotaxonomic markers for this family. Among this particular type of metabolite, pristimerin and tingenone are considered traditional medicines in Latin America. The validated method reported could be extended and be useful in analyzing Celastraceae species and real commercial samples
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
