Abstract
Background: Remdesivir is an excellent drug for the medication of covid-19.So; it is an interesting method to develop a novel and reliable HPLC-MS strategy for establishment of Remdesivir.
 Objective: To develop a new rapid and sensitive HPLC-MS/MS method for the estimation of Remdesivirin rat plasma using D4- Remdesivir.
 Methods: Separation was carried on Inertsil ODS column (150x4.6mm, 3.5µm) using a isocratic elution with a buffer containing 1ml of tri ethyl amine in 1Litre of water and the mixture of two components like Buffer and Acetonitrile in the ratio of 50:50 as mobile phase with 1ml/min flow rate at ambient temperature.
 Results: Analysis was performed within 10 minutes over a good linear concentration range from 1ng/ml to 20ng/ml(r2 = 0.999) for Remdesivir .Precision and recovery study results were within the acceptable limit. Anelectro spray ionization source was used to study Remdesivir. This method has been successfully applied; exploring Remdesivir with its internal standard (D4- Remdesivir) was extracted from rat plasma using liquid-liquid extraction.
 Conclusion: This paper focuses on the consistent evaluation of key bio-analytical validation parameters, including accuracy, precision, sensitivity, selectivity, and standard curve, quantification limits, range, recovery, and stability. In the case of chromatographic methods used in bio analysis, these validation parameters are defined, along with examples of validation methodology.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
