Abstract
Flurbiprofen (FBP) is a strong nonsteroidal anti-inflammatory drug which has analgesic, antipyretic and anti-inflammatory effects. Chlorhexidine gluconate (CHG) is a potent antibacterial agent. Nowadays, FBP and CHG as a combination are used in some pharmaceutical products. In this study, an analysis method was developed for the simultaneous determination of FBP and CHG using high performance liquid chromatography (HPLC) technique. During analysis, Agilent Poroshell 120 EC-C18 column was used as stationary phase with a guard column having the same properties. Sodium phosphate buffer solution (100 mM, pH 2.5) and acetonitrile were used as mobile phase. The chromatography was performed by gradient elution at a flow rate of 0.5 mL min−1. Analytes were detected at 248 nm using a diode array detector (DAD). The developed method was validated according to United States Pharmacopoeia. It was found to be linear in the concentration range between 1 and 25 ppm. Determination coefficient was 0.9999 for FBP and CHG. The developed method was applied successfully for HPLC analysis of commercial pharmaceutical products including FBP and CHG.
Published Version
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