Abstract
In this study, a simple, reliable and accurate method for the simultaneous separation and determination of naringin, hesperidin, neohesperidin, baicalin, wogonoside, baicalein, wogonin, emodin and chrysophanol in ‘Da-Chai-Hu-Tang’ was developed by reverse-phase high-performance liquid chromatography (RP-HPLC). The chromatographic separation was performed on an Agilent ZORBAX C18 column (250 mm × 4.6 mm i.d., 5.0 μm), and the mobile phase composed of methanol and water containing 1% (v/v) acetic acid was used to elute the targets in a gradient elution mode. The flow rate and detection wavelength were set at 0.8 ml/min and 280 nm, respectively. All calibration curves of the nine components expressed good linearities (r2≥0.9992) within the tested ranges. The RSD values demonstrated the intra- and inter-day precisions were less than 2.89%, and the recoveries of the investigated compounds were between 96.22% and 105.28%. The proposed method is simple, precise, specific, sensitive, and successfully applied to determine the nine marker compounds in ‘Da-Chai-Hu-Tang’ for quality control.
Highlights
Traditional Chinese medicines (TCMs), especially in China, have played an important role in clinical therapy, and been widely used for the prevention and treatment various diseases because of its high effectiveness and low toxicity for thousands of years [1,2].Generally, herbal medicines are used in combinations to afford a formula composed of several single herbs
The Relative standard deviation (RSD) values demonstrated the intra- and inter-day precisions were less than 2.89%, and the recoveries of the investigated compounds were between 96.22% and 105.28%
Valid and optimal chromatogram, some high-performance liquid chromatography (HPLC) analytical parameters including separation column, mobile phase and its elution mode were all investigated in this study
Summary
Traditional Chinese medicines (TCMs), especially in China, have played an important role in clinical therapy, and been widely used for the prevention and treatment various diseases because of its high effectiveness and low toxicity for thousands of years [1,2]. Herbal medicines are used in combinations to afford a formula composed of several single herbs. Some TCMs have been widely administrated directly after boiling with water without any quality assessment in some areas of China, which may produce some side effects and influence the activities of herbal products because of different herbs from different regions with different contents of active compounds. That is why the quality of TCMs is very critical important for affording the efficiency and avoiding the toxicity.
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