Abstract
Anovel and sensitive chemiluminescence (CL) method was developed and validated for the determination of citalopram in bulk dosage form and in pharmaceutical preparations. The method is based on the use of Cu 2+ -grafted oxidized multiwall carbon nanotubes (Cu/ox-MWCNTs). The weak CL signal arising fromthe reaction of an alkaline luminol-H 2 O 2 system was significantly enhanced by the addition of citalopram in the presence of ox-MWCNTs/Cu 2+ . Furthermore, ox-MWCNTs/Cu 2+ exhibited exceptional catalytic activity towards the oxidation of luminol in the luminol-H 2 O 2 CL reaction. Multiwalled carbon nanotubes (MWCNTs) were characterized by scanning electron microscopy (SEM), which also showed the attachment of Cu 2+ toMWCNTs. Various factors affecting CL intensity were carefully investigated and optimized for citalopram quantitation. The CL intensity was proportional to citalopram concentration in the range 0.2–8.0 μ g mL –1 , with a correlation coefficient of 0.996. The limit of detection (LOD) and limit of quantification were 2.29 × 10 –5 μ g mL –1 and 7.64 × 10 –5 μ g mL –1 , respectively, and its reproducibility was satisfactory with a relative standard deviation (RSD) of 2.59 % (n = 5). The interference effects of common excipients were studied, and the developed method was effectively applied for the determination of citalopram in pure formand in pharmaceutical preparations. Percentage recoveries were calculated and ranged from 98.67 to 101.46 % for the pure form and from 97.38 to 101.72 % for pharmaceutical preparations. Keywords: Chemiluminescence, Citalopram, Luminol, oxidized multiwall carbon nanotubes
Highlights
Citalopram (CIT; 1- (3-dimethylaminopropyl)-1-(4-fluorophenyl)-1, 3-dihydroisobezofuran-5-carbonitrile)[1] is a second generation selective serotonin reuptake inhibitor, which belongs to the group of drugs known as antidepressants
We found that ox-Multiwalled carbon nanotubes (MWCNTs)/Cu2+ enhanced the CL signal arising from the reaction between alkaline luminol and H2O2, and that CL intensities were further boosted by the presence of citalopram
The Pristine-MWCNTs appeared as separate thread-like carbon fibres (Fig. 2(a)), whereas ox-MWCNTs were aggregated (Fig. 2(b)), and Cu2+ coated ox-MWCNTs were more aggregated (Fig. 2(c))
Summary
Citalopram (CIT; 1- (3-dimethylaminopropyl)-1-(4-fluorophenyl)-1, 3-dihydroisobezofuran-5-carbonitrile)[1] is a second generation selective serotonin reuptake inhibitor, which belongs to the group of drugs known as antidepressants. Tion to being small sizes, CNTs have huge surface areas, hollow or layered structures, high mechanical strengths, and significant electrical conductivities These properties are of interest in various applications, such as, field emission, reinforcing materials for composites, nanoprobes, and chemical sensors.[42,43,44,45,46] CNTs are available as singled-walled carbon nanotubes or multiwall carbon nanotubes (MWCNTs) according to the number of layers carbon atom in their walls. We found that ox-MWCNTs/Cu2+ enhanced the CL signal arising from the reaction between alkaline luminol and H2O2, and that CL intensities were further boosted by the presence of citalopram Based on these observations, we developed a sensitive CL method for the quantification of citalopram in pharmaceutical preparations. CL intensity was found to be linear to citalopram concentration in the range 0.2–8.0 μg mL–1 with a detection limit of 2.29 × 10–5 μg mL–1
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
