Abstract
A new method for monitoring ivermectin content in HEARTGARD CHEWABLES® has been developed and validated. The method consists of the automated extraction of ivermectin from the meat-based formulation under conditions of elevated temperature and pressure (accelerated solvent extraction, ASE®), and determination of the active by reverse-phase high performance liquid chromatography (HPLC). The method resolves both active species of ivermectin (components H 2B 1a and H 2B 1b) from the formulation matrix.
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