Abstract
A simple, efficient and reproducible method for the simultaneous determination of Clotrimazole and its Impurity (2-Chlorphenyl diphenyl methanol) has been developed using reversed phase high performance liquid chromatography. The separation was carried out using a mobile phase consisting of methanol: acetonitrile (95:5 % v/v). Column used was Zorbax C18 (100 × 4.6 mm internal diameter) 3.5 µm with flow rate of 1 ml/min. The detection wavelength used was 225 nm. The retention time of Clotrimazole and 2-Chlorophenyl diphenyl methanol were 2.81 and 2.14 respectively. Linearity of Clotrimazole and its Impurity are in range of 10-80 µg/mL and 2-12 µg/mL respectively. Analytical parameters were calculated and a statistical evaluation included.
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