Abstract
The safety, efficacy and stability of natural antioxidants have been the focus of research in the food industry, with the aim of rapidly analyzing and controlling the quality of rosemary and its extracts, a novel analytical method involving high-performance liquid chromatography with evaporative light scattering detection (HPLC-ELSD) was developed for the simultaneous determination of rosmarinic acid, carnosol, carnosic acid, oleanolic acid and ursolic acid in rosemary. Chromatographic separation was conducted with gradient elution mode by using a Zorbax SB-C18 column (4.6 mm × 250 mm, 5 μm) with mobile phases of methanol and 0.6% acetic acid. The drift tube temperature of ELSD was 70 °C, and the pressure of nebulizer nitrogen gas was 40 Psi. The method developed has high sensitivity (with limits of detection from 1.3 to 8.6 μg/mL), acceptable linearity over the tested concentrations (with correlation coefficients from 0.991 to 0.999), good repeatability (with intra- and inter-day CV less than 3.1% for all analytes) and satisfactory accuracy (with recovery between 95.5% and 100.8%). The method has been demonstrated as a powerful tool for the functional ingredients analysis and quality control of rosemary and its extracts in a cost- and time-effective manner.
Highlights
Rosemary (Rosmarinus officinalis L.) is an aromatic evergreen perennial shrub from the Lamiaceae mint family [1], which has been widely used for a long time as a food spice and folk medicine.More than 20 varieties of rosemary plants have been found worldwide so far
Spectrum Chemical Mfg Corp (Gardena, CA, USA), acetic acid was of analytical grade and supplied from Sinopharm Chemical Reagent (Shanghai, China), high-performance liquid liquid chromatography (HPLC) grade trifluoroacetic acid was purchased from Sigma-Aldrich
A simple, sensitive and reliable quantitative method for simultaneous analysis of rosmarinic acid, carnosol, carnosic acid, oleanolic acid and ursolic acid in rosemary was developed based on HPLC-Evaporative light scattering detection (ELSD)
Summary
Rosemary (Rosmarinus officinalis L.) is an aromatic evergreen perennial shrub from the Lamiaceae mint family [1], which has been widely used for a long time as a food spice and folk medicine.More than 20 varieties of rosemary plants have been found worldwide so far. Rosemary contains a number of phytochemicals, including rosmarinic acid, carnosol, carnosic acid, oleanolic acid, ursolic acid (Figure 1). Those compounds and some of their derivatives like 3-O-acyl ursolic acid have been demonstrated to have multiple physiological activities such as antioxidant [2,3,4,5], antibacterial [2,3,6], Molecules 2019, 24, 323; doi:10.3390/molecules24020323 www.mdpi.com/journal/molecules. [10], and antithrombotic [11], [12,13], and anti-HIV effects anti-cancer anti-diabetic antithrombotic anti-HIV effects and could be and could therapeutic agent indisease neurodegenerative diseaseRosemary treatmentand [14,15,16].
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