Abstract
Herbal drugs are composed of single or several types of medicinal plants with additives. This type of preparations is gaining a wide range of popularity among a large a number of people throughout the world. With the increase of usage, it is necessary to maintain the quality of these drug preparations. Therefore, analytical approaches for their intended use in drug quality evaluation need to be validated. Our aim was to develop simple, rapid, selective, precise and economical UV-spectroscopic method for the quality control of black plum containing herbal preparation in different marketed formulation. Spectrometric analysis of black plum using ethanol shows the maximum absorbance at 279 nm. It has shown linear absorbance over the concentration range of 0.1-0.5 µg/ml with R2 value 0.9914. The developed method was validated as per ICH Q2 (R1) guidelines for various parameters namely linearity, precision, accuracy, robustness. The results were calculated statistically and it showed compliance with ICH guidelines. The relative standard deviation values and percent recovery has been found to be satisfactory, suggesting that the approach proposed is accurate. This proposed method can thus be used for the regular analytical study of black plum in bulk and pharmaceutical formulation.
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