Abstract
A simple, accurate, precise and rapid reverse phase high performance liquid chromatography method was developed and validated for the analysis of venlafaxine hydrochloride in bulk and marketed pharmaceutical dosage form. The chromatographic separation was carried out using a Phenomenex C18, (250mm × 4.6mm × 5µ) column. The optimum mobile phase consisted of ammonium acetate buffer, methanol (60:40) with pH adjusted to 5. The mobile phase was delivered isocratically at a flow rate of 1mL/min with UV detection at 227nm. Venlafaxine hydrochloride was eluted at 2 minutes. The method was validated for linearity, precision, accuracy, robustness and recovery. The method was linear in the concentration range of 15-165µg/ml with correlation coefficient 0.9997. limit of detection and limit and quantification were found to be 185ng/ml and 563ng/ml respectively.
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