Development and validation of a reversed‐phase high‐performance liquid chromatography method for quantification of hesperidin from a nutraceutical

Abstract

AbstractHesperidin is a flavonoid predominantly found in citrus fruits, primarily in orange and lemon. Recent studies report hesperidin to be a promising compound for the treatment of coronavirus disease 2019 due to its ability to bind to severe acute respiratory syndrome coronavirus 2 receptors. A global rise in hesperidin‐containing products calls for a rapid and cost‐effective method for hesperidin estimation. Bridging this gap, the present study developed a simple but efficient method for the isolation and quantification of hesperidin from a nutraceutical product by reversed‐phase high‐performance liquid chromatography. The analysis was carried out on a high‐performance liquid chromatography system using Cosmosil C18 Column (250 × 4.0 mm internal diameter; 5 μm particle size) and Methanol: Water (70:30 v/v) as the mobile phase. The injection volume was 20 μl and detection was done at 231 nm in an ultraviolet detector. The method was validated for linearity, accuracy, precision, limit of detection, limit of quantification, robustness, and ruggedness. From the validation studies, the present method was found to be specific, accurate, precise, reliable, and reproducible. Linearity was found to be R2 = 0.9997 and recovery was around 99.81% for hesperidin. The method is thus, a rapid and cost‐effective quality control tool for routine quantitative analysis of hesperidin from pharmaceuticals/nutraceuticals/citrus fruit juices/skin care products.