Abstract

L-DOPA is an antiparkinsonian drug and is used per oral application. A reliable liquid chromatographic method for determination of L-DOPA in leaves, flowers and seeds of Vicia faba L. grown in Turkish habitats and pharmaceutical dosage forms has been developed. The analysis has been carried out using Ace C18 (5 µm, 4.6 x 250 mm,) column, and the separation was carried out using a mobile phase consisting of 50 mM potassium dihydrogen phosphate (pH 2.3) at a flow rate of 1.2 mL min-1 with UV detection at 280 nm. The method has been validated according to the acceptance criteria of the ICH guidelines. The method demonstrated good linearity (R2 > 0.999) over the assayed concentration range and showed good intra-day and inter-day precision. The detection limit (LOD) and the quantification limit (LOQ) values were determined as 1.70 and 5.13 μg mL-1 for L-DOPA. This method has been successfully used for the determination of L-DOPA in pharmaceutical formulations and plant materials.

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