Development and validation of a new qualitative ELISA screening for multiresidue detection of sulfonamides in food and feed

Abstract

An indirect competitive enzyme-linked immunosorbent assay (ELISA) to screen sulfonamide residues in food (muscle, eggs, milk and honey) and feed has been developed and validated according to Commission Decision 2002/657/EC. The immunoreagents were appropriately produced to detect a wide range of sulfonamide antibiotic congeners, obtaining half-maximum inhibition concentration (IC50) values below 10 μg L−1 for 11 sulfonamides widely used in veterinary practices. Taking into account the complexity of involved matrices, specific sample preparation protocols have been optimised combining high method throughput with low detectable concentrations. Accordingly, depending on the congener, the obtained detection capabilities (CCβs) were lower or equal to 20 μg kg−1 (muscle, eggs and milk), 10 μg kg−1 (honey) and 2 mg kg−1 (feed). Finally the developed qualitative test was applied to real samples collected within the official monitoring programmes: results exceeding the established screening cut-off were re-analysed with a suitable confirmatory method. The presence of one or more sulfonamides was found in all the suspect screening samples thus demonstrating that the proposed ELISA can be successfully applied in class-specific detection of sulfonamides in food and feed.