Abstract
X-chromosome short tandem repeat (X-STR) markers are a powerful complementary system used for paternity and forensic casework. This study presents the development and validation of a new highly efficient multiplex-fluorescent-labeled 19 X-STR typing system, including DXS10079, DXS101, DXS10135, DXS10162, DXS6795, DXS6800, DXS6803, DXS6807, DXS6809, DXS6810, DXS7133, DXS7423, DXS981, DXS9902, DXS9907, GATA165B12, GATA172D05, GATA31E08 and HPRTB along with sex-typing locus, amelogenin. The system was validated according to guidelines issued by the Scientific Working Group on DNA Analysis Methods. Allele frequency and forensic parameters were investigated from 1085 (494 males and 591 females) unrelated Beijing Han individuals, the combined power of discrimination by the 19 X-STR loci in females and males, as well as the combined mean exclusion chance in trios and duos, were 0.999999999999999995, 0.99999999995, 0.9999999995, and 0.9999996, respectively. The results demonstrate that this multiplex system is robust and reliable, and considered to be a powerful tool for forensic application.
Highlights
X-chromosome short tandem repeat (X-STR) markers are a powerful complementary system used for paternity and forensic casework
We developed a new highly efficient multiplex 5 fluorescent dye labeled 19 X-STRs typing system
The multiplex typing system was validated for robustness, sensitivity, precision, sizing accuracy, species specificity, performance against DNA mixtures, and stutter according to Scientific Working Group on DNA Analysis Methods (SWGDAM) guidelines
Summary
X-chromosome short tandem repeat (X-STR) markers are a powerful complementary system used for paternity and forensic casework. Allele frequency and forensic parameters were investigated from 1085 (494 males and 591 females) unrelated Beijing Han individuals, the combined power of discrimination by the 19 X-STR loci in females and males, as well as the combined mean exclusion chance in trios and duos, were 0.999999999999999995, 0.99999999995, 0.9999999995, and 0.9999996, respectively. X-chromosomal STRs (X-STRs) can be used as a complementary tool in complex kinship analysis and deficiency c ases3,4 Allele frequency and forensic parameters were investigated from 1085 (494 males and 591 females) unrelated Beijing Han individuals. We believe that these data and the present multiplex system will prove useful in future human population genetic and forensic studies
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