Development and validation of a multianalyte method for quantification of aflatoxins and bongkrekic acid in rice and noodle products using PRiME-UHPLC-MS/MS method

Abstract

An analytical method based on PRiME (process, robustness, improvements, matrix effects, ease of use) HLB purification followed by the ultra-high liquid chromatography tandem mass spectrometry (UHPLC-MS/MS) detection has been developed for the determination aflatoxin B1, B2, G1 and G2 and bongkrekic acid in rice and noodle products. Five toxins were separated on a Waters BEH C18 column by gradient elution, scanned by ESI+ and ESI− dynamic switching and detected with MRM mode. LOD, LOQ, matrix effects, accuracy and precision of the developed method were investigated. Under the optimal sample pretreatment conditions, high sensitivity (LOQs: 0.20–0.40 μg/kg), good recoveries (80.5%-106.6%) and acceptable precision (2.4%-7.2%) were obtained for the analysis of the four aflatoxins and bongkrekic acid. This method was successfully applied to the analysis of rice and noodle products, demonstrating its applicability and suitability for the routine analysis of aflatoxins and bongkrekic acid in rice and noodle products.