Development and validation of a liquid chromatographic method for the determination of nitrofuran residues in fish samples

Abstract

A sensitive, accurate and efficient liquid chromatographic method has been developed for determination of nitrofuran residues in a honey sample; It was found that the detection minimum for all four nitrofuran group substances is 0.03 μg / ml, the specific wavelength is 376 nm, the specificity coefficient of variation for both on one working day and one working week is <2%, accuracy for solutions of three different concentrations is <2 %, The accuracy of the method for all four test substances is <2%, indicating the relevance of the method specificity to the criteria set for the analytical methods; The caliber graph for nitrofuran group is linear in the range 0.03 μg / ml - 1 μg / ml - 1 - 5 μg / ml and is acceptable as long as the correlation coefficient in all cases is> 0.999 (> 0.99); Sample preparation is satisfactory as the obtained sampling coefficient is> 0.6. Thus, as a result of validation of the liquid chromatographic method developed for the quantitative determination of nitrofuran residues in the honey sample, the method was fully complied with the requirements of the Government of Georgia according to the following validation characteristics: detection minimum, specificity, accuracy, correctness and linearity. (#499, Guidance for Industry Bioanalytical Method Validation U.S. Department of Health and Human Services Food and Drug Administration Center for Drug Evaluation and Research (CDER) Center for Veterinary Medicine (CVM) May 2001).