Abstract
A rapid and reliable HPLC-MS/MS method has been developed and validated for the simultaneous quantification of twelve bioactive compounds (baohuoside II, baohuoside I, sagittatoside A, sagittatoside B, magnoflorine, epimedin A, epimedin B, epimedin C, chlorogenic acid, neochlorogenic acid, cryptochlorogenic acid and icariin) in rat plasma. The collected plasma samples were prepared by protein precipitate with acetonitrile. The twelve compounds were separated on a CORTECS®C18 column (4.6 mm × 150 mm, 2.7 μm) with a gradient mobile phase system of 0.1% (v/v) formic acid and acetonitrile at a flow rate of 0.3 mL/min. All of the analytes were quantitated using electrospray ionization (ESI) in negative ion mode with selected reaction monitoring (SRM). The intra- and inter-day accuracy ranged from −5.6% to 13.0%, and the precisions of the analytes were less than 10.9%. The mean recoveries of the analytes were in the range of 60.66% to 99.77% and the matrix effect ranged from 93.08% to 119.84%. Stability studies proved that the analytes were stable under the tested conditions, with a relative standard deviation (RSD) lower than 11.7%. The developed method was successfully applied to evaluating the pharmacokinetic study of twelve bioactive compounds after oral administration of Epimedium extract in rat.
Highlights
IntroductionEpimedium is a traditional tonic herb used for “reinforcing the Yang and nourishing the kidney”
Epimedium is a traditional tonic herb used for “reinforcing the Yang and nourishing the kidney”in China
The developed method was successfully applied to evaluating the pharmacokinetic study of twelve bioactive compounds after oral administration of Epimedium extract in rat
Summary
Epimedium is a traditional tonic herb used for “reinforcing the Yang and nourishing the kidney”. Four species of Epimedium, including Epimedium koreanum Nakai., Epimedium pubescens. Et Zucc.) Maxim., are recorded in Chinese Pharmacopeia (Edition 2015). Epimedium are used to treat impotence, forgetfulness and neurasthenia [1]. Pharmacological study has demonstrated that Epimedium possesses a variety of activities, such as inhibition of tumor, anti-oxidation and anti-inflammatory [2,3]. Clinical research has suggested the treatment of osteoporosis, diabetes, releasing depression, cardiovascular diseases, chronic nephritis and rheumatoid arthritis with Epimedium [4,5,6,7]. Phytochemical investigation has found many kinds of compounds isolated and identified from
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