Development and Validation of a HPLC Method for Determination of Oxadiargyl Residues in Vegetables and Soil

Abstract

A standardized and validated method for the estimation of residues of oxadiargyl in green onion, cabbage and soil is reported. The substrates were extracted in acetonitrile, the extracts diluted with brine solution, partitioned in dichloromethane, cleaned up using activated charcoal and dried over anhydrous sodium sulphate. Final clear extracts were concentrated under vacuum and reconstituted in HPLC grade acetonitrile, and residues estimated by using high performance liquid chromatography equipped with PDA detector and C18 column. Acetonitrile was used as mobile phase at flow rate of 0.3 mL min−1. The average recoveries were over 85 per cent for oxadiargyl at different spiking levels. The precision (repeatability) and reproducibility) in different matrices ranged from 3.01 to 5.21 and 2.43 to 3.07 per cent, respectively. The limit of quantification was worked out to be 0.05 mg kg−1. The analytical method was validated in terms of parameters including selectivity, linearity, precision and accuracy.