Abstract
Dokhwalgisaeng-tang (DHGST) is an herbal medicine formula that is frequently used in the treatment of arthritis in Korea and consists of 16 medicinal herbs. In this study, a simultaneous analysis method for quality assessment of DHGST by universal and widely used high-performance liquid chromatography was developed and validated. Twenty-four marker components were separated on a reverse-phase SunFire C18 column (4.6 × 250 mm, particle size; 5 μm) maintained at 40 °C using a gradient elution of two mobile phase systems (0.1% aqueous formic acid and 0.1% formic acid in acetonitrile). The developed method was validated via linearity, limit of detection, limit of quantification, recovery, and precision. Using the developed method, 24 marker components in DHGST were founded at 0.23–14.68 mg/g, and this method will be used as basic data for the quality assessment of DHGST or other herbal medicine prescriptions.
Highlights
Herbal medicine prescriptions are generally composed of two or more herbal medicines and can be prepared in various formulations such as tang, pills, granules, and powders
24 marker components in DHGST were founded at 0.23–14.68 mg/g, and this method will be used as basic data for the quality assessment of DHGST or other herbal medicine prescriptions
24 components were identified as suitable markers in the DHGST sample, and these analytes were used as marker compounds for quality control of the DHGST sample in the subsequent studies
Summary
Herbal medicine prescriptions are generally composed of two or more herbal medicines and can be prepared in various formulations such as tang (decoction), pills, granules, and powders. Dokhwalgisaeng-tang (DHGST; alternatively, Duhuojisheng-tang in Chinese) is a typical herbal formula used in Korean medicine for the treatment of rheumatoid arthritis and neuritis [1]. 金要方) written by Sun Simiao in the Tang Dynasty in China, it has been included in Donguibogam (東醫寶鑑) written by Heo Jun in the Joseon Dynasty, a well-respected. Korean oriental medicine book [1,2]. According to the Donguibogam, DHGST is composed of 16 medicinal herbs (Aralia continentalis Kitag., Angelica gigas Nakai, Paeonia lactiflora Pall., Taxillus chinensis (D.C.) Danser, Rehmannia glutinosa (Gaertn.) D.C., Cnidium officinale Mak., Panax ginseng C.A. Mey., Poria cocos Wolf, Achyranthes bidentata Blume, Eucommia ulmoides. Glycyrrhiza uralensis Fisch., and Zingiber officinale Roscoe) and has been used to treat muscle cramps, bone pain, lower-back pain, and knee pain caused by liver–kidney Yin deficiency [1]
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