Development and Validation by Reverse Phase High Performance Liquid Chromatography Method for the Estimation of Piperine and Coenzyme Q10 in Bulk and Pharmaceutical Dosage Form

Abstract

Today people are focused on their health, as good health is a key to the happiness of our life. Medicine takes a big responsibility to take care of our health. Various supplements are available to meet the nutritional requirement. One such supplement is a combination of piperine and coenzyme Q10. The medicines have to be analyzed properly before introducing them to the market. As no analytical method is available for simultaneous estimation of the combination, hence an attempt was made to develop a validated method for the estimation of piperine and coenzyme Q10 simultaneously in bulk and its dosage form. A simple, precise, accurate and validated reversed phase high performance liquid chromatography technique was developed for the estimation of piperine and coenzyme Q10 in bulk and its tablet formulation. In this developed method, Waters X Bridge C18 column (250 mm×4.6 mm, 5 μm) was used as a stationary phase and acetonitrile, tetrahydrofuran and water was used in 65:32:3 (v/v) ratio as mobile phase with 1 ml/min flow rate. This isocratic separation was accomplished using Waters 2707 Autosampler high performance liquid chromatography system, Waters 515 solvent delivery system, with photodiode array detector detection at 275 nm. Chromatographic data was processed by Empower 2 software. The retention times of coenzyme Q10 and piperine were 4.56 and 8.19 min respectively. The linearity ranges have lied between 4-6 μg/ml, 240-360 μg/ml for piperine and coenzyme Q10 respectively with 0.997 as correlation coefficient for both.