Abstract
A method is described for the serological typing of Pseudomonas cepacia. Mice were immunized with each of 39 P. cepacia isolates by intraperitoneal injection of whole cells. Based upon agglutination reactions with homologous and heterologous anti-sera, the P. cepacia isolates were separated into five serotypes, A-E. Large volumes of antisera were obtained by immunization of rabbits with whole cells of each of the five type strains. These antisera were used to type 137 P. cepacia isolates from five geographic locations including consecutive isolates from individual cystic fibrosis patients. Results from these studies indicate that strains related by geographic isolation site demonstrate a tendency toward serological relatedness, and that cystic fibrosis patients tend to be infected by one P. cepacia serotype and remain infected with that same serotype over time.
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