Development and structure of four different stamens in Clematis macropetala (Ranunculaceae): particular emphasis on staminodes and staminal nectary.

Abstract

The stamens of angiosperms are diverse in number, colour and structure. The morphological and structural changes of stamens show important evolutionary significance for improving pollination efficiency. In Clematis macropetala, the androecium consists of fertile stamens and tepaloid staminodes. However, studies on the developmental features, structures and possible functions of stamens are few. In this study, the stamen ontogeny, micromorphology and nectary structure of C. macropetala were studied by scanning electron microscopy, light microscopy and transmission electron microscopy. The results indicate that the stamens can be divided into four forms according to shape and anther size: tepaloid staminode (St1), spatulate staminode (St2), linear-spatulate fertile stamen (St3) and linear fertile stamen (St4). The characteristics of stamen development are similar in the early stage but gradually differentiate in the later stage. St1 has delayed development and no anther differentiation. St2 develops abnormally at the early stage of anther differentiation. St3 and St4 are fertile, but their anther sizes are different. Nine epidermal cell types were observed in stamens, with only 4 types in St1 and 6-7 types in St2, St3 and St4. Nectary tissue appears on the adaxial side of the filament base. The nectary is composed of only one layer of secretory epidermal cells, which have a large nucleus, dense cytoplasm and well-developed wall ingrowth. Nectar is released through micro-channels in the cuticle of the outer wall. In Ranunculaceae, the staminal nectary is often located on fertile or sterile stamens, and the position, structure and micromorphology of secretory tissues of the stamen within Ranunculales are discussed.