Abstract

Gene editing technology, as an important technology in the field of science, can accurately and stably modify the genome sequence, bringing revolutionary changes to various fields of life sciences, so it has attracted wide attention from researchers in various countries. This article makes a brief introduction to ZFN and TALEN technology, and at the same time makes a detailed introduction to CRISPR/Cas9 technology, focusing on the limitations, application, and development of CRISPR/Cas9 system. This research found that the CRISPR/Cas9 system and its related technologies are going deep into various fields of life sciences and have achieved remarkable results, providing more ideas and possibilities for human disease treatment, crop gene control and other fields.

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