Abstract
The purpose of this study was to develop, optimize, and fully validate a high-sensitivity methodology using UHPLC-MS/MS to simultaneously quantify hesperidin and naringenin in microsamples (100 µL) of murine plasma after intragastric administration of single pure flavonoids and a mixture. The optimization process allowed for high sensitivity with detection limits of approximately picogram order using an electrospray ionization (ESI) source in negative mode and an experiment based on multiple reaction monitoring (MRM). The validation parameters showed excellent linearity and detection limits, with a precision of less than 8% and a recovery of over 90%. This methodology was applied to compare the pharmacokinetic parameters for the administration of hesperidin and naringenin in individual form or in the form of a mixture. The results showed an absence of significant effects (p > 0.05) for Tmax and Cmax; however, the AUC presented significant differences (p < 0.05) for both flavonoids when administered as a mixture, showing an improved absorption ratio for both flavonoids.
Highlights
Flavonoids are a selected group of polyphenolic compounds widely contained in citrus fruits; according with Tripoli and coworkers [1] cover over 60 types have been identified
The aim of our work is to develop a high sensitivity analytical methodology using High-performance liquid chromatography (HPLC)-mass spectrometry (MS)/MS with detection limits in the picogram-order to extract hesperidin and naringenin from rat plasma
For UHPLC-MS systems, the flow rates range between 200 μL/min to 500 μL/min; the electrospray ionization (ESI)-jet stream allows for flow rates of approximately
Summary
Flavonoids are a selected group of polyphenolic compounds widely contained in citrus fruits; according with Tripoli and coworkers [1] cover over 60 types have been identified. The main classification of these compounds are as glycosides (neohesperidosides and rutinosides) and aglycones as naringenin and hesperetin [1]. A recent investigation demonstrated that naringenin increases locomotor activity and reduces diacylglycerol accumulation [2]; hesperidin affects the microbial spoilage and antioxidant quality [3]. Recent research has focused on studying a mixture with two or more flavonoids to increase or improve the pharmacological effect. A hesperidin/naringenin mixture exhibits vasorelaxant and antihypertensive properties [8], antioxidant activity, and a membrane phospholipid composition [9], Molecules 2020, 25, 4241; doi:10.3390/molecules25184241 www.mdpi.com/journal/molecules
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
