Development and inter-laboratory study of a method for quantification of fumonisin B1, B2 and B3 in pet foods

Abstract

An analytical method to determine fumonisin B1 (FB1), B2 (FB2) and B3 (FB3) in pet foods using a liquid chromatography-electrospray ionization-mass spectrometer (LC-ESI-MS) was developed, and an inter-laboratory study was conducted in eleven laboratories. FB1, FB2 and FB3 were extracted with aqueous acetonitrile. The extract was purified by a multifunctional column, MultiSep 211 Fum, and analysed by LC-ESI-MS. The limit of quantification of fumonisins was estimated to be 0.2 mg/kg for dry and semi-dry pet foods, and 0.1 mg/kg for wet pet food. The calibration curve showed linearity in the range of 0.1-5 ng of fumonisins (0.02-1.0 μg/ml). The values of the mean recovery for FB1 at 0.1-1.0 mg/kg were 93.3-107% and of the relative standard deviation less than 7.9%. These values were 87.3-102 and 8.6% for FB2 and 90.8-102 and 8.6% for FB3, respectively. The mean recovery, repeatability, reproducibility and the Horwitz ratio for FB1 from the inter-laboratory validation study were 92.9-98.9%, 2.6-4.6%, 6.8-10% and 0.41-0.54, respectively. The values for FB2 were 91.5-94.7%, 2.7-5.9%, 6.8-8.9% and 0.33-0.55, respectively, and the values for FB3 were 90.1-94.3%, 3.3-5.9%, 7.3-9.5% and 0.44-0.57, respectively.