Abstract
A2254 and G2254 genotypes, together with identifying three cases of dual infection (A2254+G2254). In comparison, the E2 produced 20 false dual positive results and one confirmed A2254+G2254 genotype (Table 1). This increased sensitivity of E1 probably results from this assay having a shorter amplicon length compared to E2, resulting in elevated efficiency and/or less susceptibility to secondary DNA structural effects. The findings of this study demonstrate that E1 is significantly more sensitive for the diagnosis of EHV-1 and less prone to produce false dual positive results than E2. E1 is clearly a reliable and sensitive means for the detection and A2254/G2254 genotyping of EHV-1,making this improved rPCR assay a very valuable diagnostic tool when investigating outbreaks of EHV-1 infection.
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