Development and genetic diversity of X-chromosomal STR multiplex PCR systems

Abstract

Objective To learn about the genetic diversity of X-STR and to collect data of population genetics, the six X-STR loci were tested. Methods DXS6801、 DXS9902、 DXS6809、 DXS6803、 DXS6804 and DXS6799 were amplified in a single PCR reaction. PCR products were analyzed using capillary electrophoresis and ABI prism 3100 Genetic Analyzer, with GeneMapper ID 3. 1 Analysis Software. Results Allele typing with the systems was successful for all of six loci. When 202 unrelated male and 62 unrelated female individuals from Guangdong Han population were tested, 8,6, 11, 10,6 and 9 alleles were detected for DXS6801, DXS9902, DXS6809, DXS6803, DXS6804 and DXS6799, respectively. Polymorphism information content is 0. 662 3～0. 837 6. Power of discrimination in females is 0. 824 2～0. 951 1. Mean exclusion chance for X-STR in standard trios with daughters is 0. 594 9～0. 817 4. Conclusion The six loci in the multiplex system provide high polymorphism information for forensic identification and paternity testing, particularly for difficult paternity deficiency cases Key words: X-STR; Fluorescent multiplex PCR; Genetic polymorphism; Guangdong Han populationb