Development and GBS-genotyping of introgression lines (ILs) using two wild species of rice, O. meridionalis and O. rufipogon, in a common recurrent parent, O.sativa cv. Curinga.

Juan D Arbelaez,Mathias Lorieux,César P Martinez,Yolima Ospina,Namrata Singh,Lyza G Maron,Cécile Grenier,Susan Mccouch,Chih-Wei Tung,Laura T Moreno

doi:10.1007/s11032-015-0276-7

Abstract

Two populations of interspecific introgression lines (ILs) in a common recurrent parent were developed for use in pre-breeding and QTL mapping. The ILs were derived from crosses between cv Curinga, a tropical japonica upland cultivar, and two different wild donors, Oryza meridionalis Ng. accession (W2112) and Oryza rufipogon Griff. accession (IRGC 105491). The lines were genotyped using genotyping-by-sequencing (GBS) and SSRs. The 32 Curinga/O. meridionalis ILs contain 76.73 % of the donor genome in individual introgressed segments, and each line has an average of 94.9 % recurrent parent genome. The 48 Curinga/O. rufipogon ILs collectively contain 97.6 % of the donor genome with an average of 89.9 % recurrent parent genome per line. To confirm that these populations were segregating for traits of interest, they were phenotyped for pericarp color in the greenhouse and for four agronomic traits—days to flowering, plant height, number of tillers, and number of panicles—in an upland field environment. Seeds from these IL libraries and the accompanying GBS datasets are publicly available and represent valuable genetic resources for exploring the genetics and breeding potential of rice wild relatives.Electronic supplementary materialThe online version of this article (doi:10.1007/s11032-015-0276-7) contains supplementary material, which is available to authorized users.

Highlights

Asian rice (Oryza sativa L.) is the staple crop for 3 billion people around the world (Food and Agricultural Organization 2003; Bouman et al 2007)
We report the development of two sets of interspecific introgression lines (ILs) using a drought-tolerant tropical japonica cultivar, Curinga (CUR), as the recurrent parent and two wild donor accessions from the species O. meridionalis (MER) and O. rufipogon (RUF)
We demonstrate that the IL populations segregate for traits of interest, and we identified a superior IL, RUF27, that has a higher number of tillers and number of panicles than CUR under upland soil conditions

Summary

Introduction

Asian rice (Oryza sativa L.) is the staple crop for 3 billion people around the world (Food and Agricultural Organization 2003; Bouman et al 2007). To meet the growing demand for food driven by population growth and economic development, global rice production must double by 2050 (Ray et al 2013) Much of this increase is expected to come from new crop varieties that are high yielding, resource-use efficient and resistant to diseases, insects and abiotic stresses, problems that are exacerbated by climate volatility (Godfray et al 2010; Tester and Langridge 2010). Wild genetic diversity is most effectively tapped for crop improvement through the creation of interspecific populations via backcrossing with a well-adapted and productive cultivar—a practice that is often termed ‘pre-breeding’ This process is labor and time-consuming and typically fraught with difficulties due to incompatibility barriers, limited recombination, and linkage drag, all of which limit the ease of identifying, transferring, and utilizing beneficial wild alleles in crop improvement (Lorieux et al 2004; Brar and Khush 1997; Tanksley and McCouch 1997)

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