Abstract
Biofertilizer is a group of beneficial microorganisms used for improving the productivity of soil by fixing atmospheric nitrogen or by solubilizing soil phosphorus. They also stimulate plant growth through synthesis of growth promoting substances. In this present study, Azospirillum lipoferum is grown in Nitrogen free Bromothymol blue (Nfb) medium and Pseudomonas fluorescens in King’s B medium. Bioprocess condition was optimized for both of the culture and found that Pseudomonas fluorescens has shown highest growth at 30 0 C in pH 8 after 72 hours of incubation where as Azospirillum lipoferum showed highest cell concentration at 31 0 C in pH 7, with incubation period of 72 hours. The optimized culture is mixed with different formulations of powder and liquid carrier such as Saw dust, Rice husk, Date seed powder, Matka khad, Jiwamrit and Beejamrit respectively. Shelf life study for 0, 30, 60, 90 and 120 days by cell counting and spread plate method showed that shelf life of the biofertilizer produced from Powder and liquid carriers had high amount of viable microbial population up to 120 days storage. Among biofertilizer based bio inoculants, Saw dust showed maximum population of 77x10 9 cfu/ml for Azospirillum lipoferum and 72 x 10 9 CFU/ml for Pseudomonas strain on 120 th day and the liquid carrier Matka khad showed 85x10 9 cfu/ml for Azospirillum lipoferum and 78 x 10 9 CFU/ml for Pseudomonas fluorescens.
Highlights
Biofertilizers are the bioinoculants of specific beneficial microorganisms that promote the growth of plant crops by converting the unavailable form of nutrients into available form
Azospirillum lipoferum was maintained on Nitrogen Free Bromothymol blue
The culture of Azopirllum lipoferum and Pseudomonas fluorescens were maintained in Nitrogen free Bromothymol blue (Nfb) medium and King’s B medium respectively
Summary
Biofertilizers are the bioinoculants of specific beneficial microorganisms that promote the growth of plant crops by converting the unavailable form of nutrients into available form. These biofertilizers induce resistance in plants against pests, to improve soil fertility, to help plant growth by increasing the number and biological activity of desired microorganisms in the root surface (SIVASAKTHIVELAN et al, 2013). Bacteria produces growth-promoting substances like indole acetic acid (IAA), gibberellins, pantothenic acid, thiamine and niacin and it increases the rootlet density and root branching resulting in the increased uptake of mineral and water (VIJENDRAKUMAR et al, 2014). Production of growth promoting substances influence root development by increased uptake of nutrients from the land, and inhibiting pathogenic fungi and bacteria in the rhizosphere. Production of growth promoting substances influence root development by increased uptake of nutrients from the land, and inhibiting pathogenic fungi and bacteria in the rhizosphere. (HOSSAIN ET al., 2015)
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