Abstract
Siniperca chuatsi rhabdovirus (SCRV) is a rod- or bullet-shaped negative-stranded RNA virus and a threat to mandarin fish farming. This virus naturally infects fish, such as S. chuatsi and Micropterus salmoides, among others. SCRV-infected fish have a 90% mortality rate. In this study, a recombinant SCRV-N protein and a polyclonal antibody were prepared by gene cloning, constructing the vector of the SCRV nucleoprotein gene, purifying the protein, and immunizing mice with the recombinant nucleoprotein. A SCRV-N competitive enzyme-linked immunosorbent assay was established based on the recombinant protein and polyclonal antibody and verified by a large number of experimental fish infected with SCRV. The results confirmed that the method established in this study can be used to confirm whether fish are infected with SCRV with high sensitivity and speed. This rapid detection test is suitable for large-scale applications and will provide support for diagnosing infection with the mandarin fish rhabdovirus.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
