Abstract
This work was to report preparation and quality control of targeted microbubbles (MBt) via biotin–avidin bridge, specifically verification of the “biotin–avidin–biotin” sandwich structure. (1) Lipid microbubbles filled with sulphur hexafluoride were produced by sonication and compared with commercially available microbubbles. (2) MBt were produced via biotin–avidin bridge. Specifically, the “biotin–avidin–biotin” sandwich structure on the surface of MBt was verified in vitro using fluorescence. (3) Adhesion of alpha(v)beta(3)-integrin targeted MBt (MBαvβ3) to human umbilical vein endothelial cells (HUVECs) was tested using the parallel plate flow chamber (PPFC). “Biotin–avidin” is a reliable method to attach molecular probe onto the surface of microbubbles.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
