Abstract
A colloidal gold immunochromatographic test strip was firstly developed to detect Canine Parainfluenza Virus type 5 based on a sandwich format. The sensitivity of strip was tested for 10 3.8 TCID 50 mL-1 to CPIV 5. No cross-reactivity with five common viruses from dogs were observed. Thirty-nine nasopharyngeal swabs samples were used to compare the consistency between RT-PCR and the strip, and the total coincidence rate was 87.18%. The colloidal gold immunochromatographic test strip thus provides a rapid and convenient method for on-site detection of CPIV 5 without any ancillary equipment.
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