Abstract
Public databases of SNP markers and microfluidics technology may provide the tools to rapidly assess genetic diversity. We developed and tested a cichlid fish SNP panel derived from loci in GenBank’s dbSNP database and used a Fluidigm EP-1 System for genotyping. Markers were tested on both Salton Sea Tilapia and East African species. Successful amplifications were obtained for 66% of the loci, and 54% of loci screened were variable either within or between lineages. Loci reflected an ascertainment bias favoring the taxon they were initially developed in.
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