Abstract
ObjectiveThe genetic markers designed for this study can facilitate future genetic studies on the rock ptarmigan (Lagopus muta). To our knowledge no microsatellite markers have ever been developed specifically for this species before. These new microsatellite markers will be useful for population genetics studies and for future conservation projects.ResultsUsing Next Generation Sequencing 6252 potential microsatellite sequences were found. Sixteen nonpalindromic tetranucleotide microsatellites and their respective primers were selected. The markers were tested on both the rock ptarmigan and the willow grouse (L. lagopus). The number of alleles varied between 2 and 18 for the rock ptarmigan, and between 3 and 13 for the willow grouse. Expected heterozygosity was in the range 0.1244–0.8692 and 0.1358–0.8722 for the rock ptarmigan and the willow grouse, respectively.
Highlights
The rock ptarmigan (Lagopus muta) and the willow grouse (L. lagopus) are two closely related bird species that inhabit the alpine zone throughout the northern hemisphere
In Scandinavia they are important game species. Both have experienced a drastic decline in recent years [1] and there is an urgent need for tools to investigate the underlying causes of the decline, including markers for population genetic studies
After the Titanium 454 sequencing 31037 sequences were obtained, which resulted in 6252 potential microsatellite sequences after screening with msatcommander
Summary
Abstract: The genetic markers designed for this study can facilitate future genetic studies on the rock ptarmigan (Lagopus muta). To our knowledge no microsatellite markers have ever been developed for this species before. These new microsatellite markers will be useful for population genetics studies and for future conservation projects
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