Development and characterization of two new Triticum aestivum–Dasypyrum villosum Robertsonian translocation lines T1DS·1V#3L and T1DL·1V#3S and their effect on grain quality

Abstract

Dasypyrum villosum (L.) Candargy is a diploid, wild relative of bread wheat (Triticum aestivum L.). Previous studies showed that D. villosum chromosome 1V has genes that encode seed storage proteins that may be used to enhance the grain quality of bread wheat. As a first step in genetic transfer, the present study was initiated to develop compensating Robertsonian translocations involving wheat chromosome 1D and D. villosum chromosome 1V and to analyze their effects on grain quality. A monosomic 1D stock was crossed with the disomic addition stock DA1V#3 and the double monosomic plants (20″ + 1D′ + 1V#3′) were self pollinated. Two co-dominant STS markers (BE499250 and BE591682) polymorphic for the short arm of 1V#3S and two dominant STS markers (BE518358 and BE585781) polymorphic for the long arm of 1V#3L were developed to screen a large number of progeny to identify plants that had only the 1V#3S or 1V#3L arms. Five compensating Robertsonian heterozygous translocations, two (plants #56 and #83) for the short arm (T1DL·1V#3S) and three (plants #7, #123, and #208) for the long arm (T1DS·1V#3L) were identified from 282 F2 plants and confirmed by genomic in situ hybridization and C-banding analyses. Two homozygous translocations T1DL·1V#3S (plants #14 and #39) were identified from 52 F3 plants derived from F2 plant #83. Four homozygous translocations T1DS·1V#3L (plants #3, #22, #29, and #30) were identified from 68 F3 plants derived from F2 plant #208. The homozygous translocation T1DL·1V#3S had a significantly higher (37.4 ml) and T1DS·1V#3L had significantly lower (10 ml) Zeleny sedimentation values compared to Chinese Spring wheat (30.7 ml). Our results showed that 1V#3S increased gluten strength and enhanced wheat quality, but 1V#3L decreased gluten strength and did not enhance wheat quality.