Abstract

The purpleback flying squid (Symplectoteuthis oualaniensis) is a pelagic squid that is facing the serious threat of large-scale commercial exploitation in the South China Sea. In this study, microsatellites were developed for the first time in this species using biotinylated probes for hybridization and library screening. Twenty-three polymorphic microsatellite markers were isolated and characterized in a wild population. The number of alleles varied from 5 to 28, and the observed and expected heterozygosity ranged from 0.220 to 0.867 and 0.374 to 0.958, respectively. Significant deviations from Hardy–Weinberg equilibrium values were detected in six loci. These markers will facilitate further studies in conservation of genetic diversity and sustainable utilization of S. oualaniensis.

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