Development and characterization of twelve microsatellite loci for Amolops ricketti using next-generation sequencing.

Abstract

Amphibian populations have decreased dramatically because of human disturbance. To better conserve and manage native or wild populations of Amolops ricketti, 12 novel microsatellite loci were developed using high-throughput sequencing. The number of alleles ranged from 2 to 19, with an average of 7.67 alleles per locus. The observed and expected heterozygosity ranged from 0.42 to 1.00 (average 0.81) and from 0.51 to 0.95 (average 0.76), respectively. The polymorphism information content per locus ranged from 0.38 to 0.92 (average 0.69). These polymorphic microsatellite markers will contribute to the assessment of population structure, gene flow and genetic differentiation in A. ricketti.