Abstract
Osteochilus salsburyi (Cypriniformes, Cyprinidae) is a small-sized fish of significant economic value. In this study, thirteen polymorphic microsatellite markers were developed in O. salsburyi and were examined on twenty individuals collected from the Wanquan River in the Hainan Island. The number of alleles (NA), polymorphism information content, observed and expected heterozygosity per locus ranged from 10 to 21, 0.8020 to 0.9354, 0.7500 to 1.0000 and 0.8436 to 0.9628, respectively. No locus was found to significantly deviate from Hardy–Weinberg equilibrium. These markers would be suitable for studies on conservation genetics and population structure of O. salsburyi.
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