Development and characterization of porous functionalized collagen scaffolds for delivery of FGF-2

Ia O Pokholenko,I Ya Dubey,O V Moshynets,M D Chetyrkina,M I Degtiarova,V A Kordium,S P Shpylova,E V Sheludko,L V Dubey

doi:10.7124/bc.000899

Abstract

Aim. To develop the porous functionalized collagen scaffold for the delivery of FGF-2 and studying its properties in vitro and in vivo. Methods. Porous collagen scaffolds were prepared by freeze- drying collagen I solutions containing the polymer developed on the basis of cross-linked modified heparin. The scaffolds have been analyzed by SEM, AFM and SCLM. The angiogenic activity of these scaffolds loaded with FGF-2 was tested in a CAM assay. Results. The data obtained by SEM and SCLM analysis revealed that the scaffold mainly hasalayeredstructurewithporesformingaconnection between the layers. The average pore size of the scaffolds varied from 76 to 150 µm. Scaffolds containing the polymer were able to incorporate human FGF-2. Proposed compositions promoted angiogenesis in CAM assay. Conclusions. The developed porous functionalized collagen scaffold incorporating FGF-2 can be used as a vehicle for the sustained delivery of the growth factor both in vitro and in vivo.

Highlights

A variety of human pathologies such as critical limb ischemia, venous stasis, stroke, angina, infarction, diabetic ulcers, etc. are the consequences of severe blood flow violation resulted in the tissue ischemia
The binding of FGF-2 to heparin sulfate proteoglycans on the cell surface serves as a mechanism for creating storage site for the proteins, from which they could be released when needed
We have developed a convenient protocol for its cross-linking, based on the reaction of heparin with a known bifunctional reagent, adipic dihydrazide (10– 20 % by weight), in the presence of water-soluble carbodiimide in aqueous medium at slightly acidic pH

Summary

Introduction

A variety of human pathologies such as critical limb ischemia, venous stasis, stroke, angina, infarction, diabetic ulcers, etc. are the consequences of severe blood flow violation resulted in the tissue ischemia. Are the consequences of severe blood flow violation resulted in the tissue ischemia. A variety of human pathologies such as critical limb ischemia, venous stasis, stroke, angina, infarction, diabetic ulcers, etc. These pathologies are among the major causes of human morbidity and mortality. According to different estimations, the peripheral arterial disease alone affects up to 10 % of people worldwide, rising to 15–20 % in humans after 70 years. In Europe and North America around 27 million people are affected [1]. The existing therapeutic and surgical approaches for the correction of vessel beds do not always result in a proper reconstitution of the blood flow, and, as a consequence, fail to restore the functional state of the damaged tissues.

Methods

Results

Conclusion