Abstract
Dactylorhiza hatagirea (D. Don) Soo is a critically endangered orchid distributed in eastern China and central Asia. In order to investigate its genetic diversity within and between populations and design effective conservation strategies, we developed microsatellite marker for this species in the present study. A total of 14 polymorphic markers were isolated and characterized using 68 individuals from three natural populations. The number of alleles per locus for a single population ranged from 2 to 15, and the polymorphism information content ranged from 0.043 to 0.908. No genotypic linkage disequilibrium within populations was detected. These microsatellite markers are the first characterized for D. hatagirea and they are expected to be useful for population level studies in this species.
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