Abstract
The peach fruit moth Carposina sasakii is an economically important pest on dozens of fruits from Rosaceae and Rhamnaceae in Northeast Asia. We developed novel microsatellite markers for C. sasakii from randomly sequenced regions of the genome using next-generation sequencing. In total, 95,153 microsatellite markers were isolated from 4.70 GB genomic sequences. Thirty-five polymorphic markers were developed by assessing in 63 individuals from two geographical populations. The allele numbers ranged from 2 to 9 with an average value of 4.60 per locus, while the polymorphism information content ranged from 0.075 to 0.696 with an average value of 0.407. Furthermore, the observed and expected heterozygosity varied from 0.000 to 0.677 and 0.062 to 0.771, respectively. The microsatellites developed provide abundant molecular markers for investigating genetic structure, genetic diversity, and existence of host-plant associated biotypes of C. sasakii.
Highlights
The peach fruit moth Carposina sasakii Matsumura (Lepidoptera: Carposinidae), is an important orchard pest in Northeast Asia [1,2]
Hua et al (1995) [11] reported that there is nearly no differentiation in isozyme-spectra between C. sasakii collected from jujube and wild jujube; populations collected from above two hosts were obviously different from those collected from apple orchard
Raw data sequences were submitted to the National Center for Biotechnology Information (NCBI) Short Read Archive under accession number SRP068817
Summary
The peach fruit moth Carposina sasakii Matsumura (Lepidoptera: Carposinidae), is an important orchard pest in Northeast Asia [1,2]. Its larvae can inflict direct damage on dozens of fruits, including peach, apple, pear, jujube, wild jujube, apricot, hawthorn, and pomegranate [3,4,5,6,7] by boring into fruitage. Hua et al (1995) [11] reported that there is nearly no differentiation in isozyme-spectra between C. sasakii collected from jujube and wild jujube; populations collected from above two hosts were obviously different from those collected from apple orchard. Using RAPD (random amplified polymorphic DNA) to compare the populations collected from six kinds of host plants, including apple, hawthorn, peach, apricot, jujube, and wild jujube, Xu and
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