Abstract
Based on RNA sequences using transcriptome analysis, 37 new simple sequence repeat (SSR) primer sets were developed for Perilla species. These new SSR markers were applied to analyze the genetic diversity among 15 accessions of Perilla species. A total of 182 alleles were confirmed in 37 loci, with an average of 4.9 alleles per locus and from 2 to 9 alleles per locus. The MAF (major allele frequency) per locus varied from 0.200 to 0.733, with an average of 0.463. The gene diversity (GD) ranged from 0.391 to 0.853, with an average of 0.670. The average polymorphic information content (PIC) was 0.624, ranging from 0.315 to 0.838. The new SSR markers of Perilla species reported in this study may provide potential markers to analyze the genetic diversity and genetic relationships of Perilla species. In addition, new Perilla SSR markers developed from transcriptome analysis can be useful for the identification of cultivars, conservation of Perilla germplasm resources, and genetic mapping and designating of important genes/QTLs for future Perilla crop breeding programs.
Highlights
Perilla frutescens (L.) Britton is widely cultivated in East Asia
New Perilla simple sequence repeat (SSR) markers developed from transcriptome analysis can be useful for the identification of cultivars, conservation of Perilla germplasm resources, and genetic mapping and designating of important genes/QTLs for future Perilla crop breeding programs
Var. frutescens is used as an oil crop, whereas var. crispa is used as a vegetable crop
Summary
Perilla frutescens (L.) Britton is widely cultivated in East Asia. There are two varieties based on the uses and morphology, P. frutescens var. frutescens and var. crispa. In East Asia, the wild species of these two varieties of Perilla crop are unknown but weedy plants of two cultivated types of P. frutescens have been identified [1] [2] [3]. RAPD (random amplified polymorphic DNA), AFLP (amplified fragment length polymorphism), and SSR (simple sequence repeat) analyses showed that the two weedy types of Perilla crop were each grouped with the two cultivated types of var. We successfully developed SSR primers from Perilla species, and these novel additional SSR markers can be used to analyze the genetic diversity and genetic relationships and to perform QTL mapping among two cultivated types of Perilla crop and their weedy types
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