Abstract

A modified method, in which explants were grown directly on glass coverslips in Leighton tubes, was employed for growing myelinated neuronal processes in tissue cultures of canine cerebellum. Myelination occurred in 60 to 75 per cent of the prepared cultures. Myelin sheaths, which were composed of distinct segments of variable length, regularly appeared after 17 to 28 days of growth. The myelin sheaths first developed along the central or proximal part of the neuronal process and became thicker with age.

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