Abstract
Campomanesia xanthocarpa is a native tree, of common occurrence in almost all Brazilian Forest formations, which has its fruits and timber with high commercial value. Using an enriched genomic library we isolated and characterized microsatellite loci for C. xanthocarpa (Myrtaceae), in order to estimate genetic diversity parameters for this and related species. Twenty-eight microsatellite loci were identified and ten of them successfully amplified and showed polymorphism in a sample of 96 individuals, from four natural populations. The number of alleles per locus ranged from two to eight, and the observed and expected heterozygosities varied from 0.042 to 1.000 and from 0.294 to 0.855, respectively. These markers were tested and validated in two related species ( C. eugenioides and C. guazumifolia ). The microsatellite markers will be used in further studies of population genetics of C. xanthocarpa , in order to understand the genetic variability and to define the strategies needed for the conservation of the species.
Highlights
Genomic DNA (5 μg) from C. xanthocarpa was digested with RsaI (Promega, Madison, USA) and blunt-ended fragments were linked to adapters Rsa-21 and Rsa-25 (Edwards, Barker, Daly, Jones, & Karp, 1996)
The genotyping of 96 individuals of C. xanthocarpa identified a total of 61 alleles, with the sizes of the fragments varying from 114 (Cxan2) to 330 bp (Cxan1) (Table 2)
We developed 28 microsatellite markers for the tree species C. xanthocarpa, 10 of which were polymorphic
Summary
Berg (Myrtaceae) is a tree species commonly known as Gabirobeira or just Gabiroba, which can reach from 10 to 20 m high with an elongated and dense top (Lorenzi, 2002). This species occurs naturally in Brazil in almost all forest formation and it can be found in Uruguay, Argentina and Paraguay (Biavatti et al, 2004). Campomanesia xanthocarpa produces a large quantity of seeds that are widely disseminated by the avifauna. The species presents medicinal value in the fight against dysentery, fever, scurvy, and urinary tract diseases (Cravo, 1994; Alice, Siqueira, Mentz, Silva, & José, 1995)
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