Abstract
Purpose: To investigate the development of mammary tumours in female Sprague-dawley rats through a simple subcutaneous injection of human adenocarcinoma breast cells (MCF7) in combination with basement membrane matrix (BME).Methods: Nine Sprague-Dawley rats were divided into three groups. Group A received no injection, group B was injected with MCF7 cells at a cell density of 7.2 x 106/ml, and group C was co-injected with MCF7 and BME at 7.2 x 106/ml and 3.158 mg/ml, respectively. Tumour growth was observed over a duration of 70 days. Hematological analysis was performed using differential blood cell counts. Histological evaluation was carried out using standard LM techniques and H&E staining.Results: At day 35, RBC concentration across all groups was 8.10 x 106/mm3, whereas by day 70 the range decreased to (7.64 – 7.87) x 106/mm3. White blood cells (WBCs) were within normal range up to day 35, but monocytes and lymphocytes displayed an increase in concentration for group C. Mammary tissues from the thoracic region showed evidence of MCF7 cellular proliferation in both groups B and C.Conclusion: This study reveals that BME enhances tumour growth. Further studies are required to investigate optimization strategies for the development of mammary tumours in alternative recipient animal.Keywords: Tumour induction, MCF7, Histopathology, Thoracic mammary gland, Mammary tumour, Basement membrane matrix
Highlights
The study of cancer is currently one of the most widely studied and dynamic fields in the forefront of animal research [1,2]
Other chemical inducers of tumours include the administration of high doses of growth hormones, oestrogens or aminofluorene compounds that stimulate the development of mammary tumours [8]
The development of rapid and efficient growth rate in xenograft techniques is achieved through the application of basement membrane extract (BME) [10,11,12]
Summary
The study of cancer is currently one of the most widely studied and dynamic fields in the forefront of animal research [1,2]. The human breast adenocarcinoma adherent cell line (MCF7) and is currently is one of the most predominant cell lines used to study breast cancer cell biology in both, in vitro and in vivo experiments [3] In addition this cell line is well characterised and a suitable choice for breast cancer research involving tumour growth, hormone and other targeted drug related therapies. Numerous studies have been conducted to demonstrate the ability of BME to increase uniform tumourigenicity in xenograft applications Most of these studies were performed on nude mice to induce mammary, pancreatic, prostate, lung and colorectal cancer tumours [10,11,12,13,14]. These animals are immunocompromised and require specialized breeding, handling and application protocols [11,13]
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