Development and Characterization of Gentamicin-Loaded Arabinoxylan-Sodium Alginate Films as Antibacterial Wound Dressing.

Abdulaziz I Alzarea,Anton V Dolzhenko,Naveed Ahmad,Muhammad Syafiq Bin Shahari,Adeel Masood Butt,Nabil K Alruwaili,Saad S Alqahtani,Muhammad Masood Ahmad,Ziyad A Alrowaili,Ziyad S Almalki,Muhammad Usman Munir

doi:10.3390/ijms23052899

Abstract

Biopolymer-based antibacterial films are attractive materials for wound dressing application because they possess chemical, mechanical, exudate absorption, drug delivery, antibacterial, and biocompatible properties required to support wound healing. Herein, we fabricated and characterized films composed of arabinoxylan (AX) and sodium alginate (SA) loaded with gentamicin sulfate (GS) for application as a wound dressing. The FTIR, XRD, and thermal analyses show that AX, SA, and GS interacted through hydrogen bonding and were thermally stable. The AXSA film displays desirable wound dressing characteristics: transparency, uniform thickness, smooth surface morphology, tensile strength similar to human skin, mild water/exudate uptake capacity, water transmission rate suitable for wound dressing, and excellent cytocompatibility. In Franz diffusion release studies, >80% GS was released from AXSA films in two phases in 24 h following the Fickian diffusion mechanism. In disk diffusion assay, the AXSA films demonstrated excellent antibacterial effect against E.coli, S. aureus, and P. aeruginosa. Overall, the findings suggest that GS-loaded AXSA films hold potential for further development as antibacterial wound dressing material.

Highlights

A wound can be defined as a breakage in intact tissue, caused by physical, thermal, chemical, or mechanical trauma, or may result from complicated pathological conditions [1,2]
These results demonstrated that the dried films were transparent but not flexible
The findings of this study demonstrate that the optimized AXSA films possess various desirable characteristics for antibacterial wound dressings

Summary

Introduction

A wound can be defined as a breakage in intact tissue ( skin), caused by physical, thermal, chemical, or mechanical trauma, or may result from complicated pathological conditions [1,2]. The complete duration of healing may vary depending upon the nature of the wound [7,8]. Depending on the time required for complete healing, wounds can be classified into acute (1–12 weeks) and chronic (>12 weeks) wounds [8,9]. Wound infections caused by bacteria (mainly Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa, Bacteroides fragilis) are one of the most predominant reasons for the prolongation of wound healing (in the inflammatory stage) [1,11–13]. These bacteria form colonies at the wound site and may overcome the patient’s immunity, resulting in tissue damage and life-threatening consequences [14,15]. The development of an active dressing may prevent wound infections and augment the healing process

Methods

Results

Conclusion