Abstract
Abstract In this study, an immunoaffinity column was prepared by covalently coupling of polyclonal antibody against danofloxacin (DAN) with chitosan microparticles and its potential application to selective extraction of DAN from food samples was also evaluated. The produced antibody exhibited high sensitivity, with an IC50 value of 4.56 ng/mL towards DAN, and good specificity, with less than 0.5% of cross-reactivity towards DAN structurally related compounds. The developed immunoaffinity column had an approximate binding capacity of 2300 ng/mL gel and a relative standard deviation (RSD) of 3.7% among columns. The immunoaffinity columns were then challenged with DAN-fortified milk samples, recoveries of DAN were found to be in the range of 83.8% to 98.8%, indicating the prepared immunoaffinity column is suitable for use as an efficient extraction method to detect DAN residues in food products.
Highlights
Danofloxacin (DAN), one of the most important fluoroquinolones, has been widely used as veterinary medicines for farm animals to prevent and treat various infection diseases
The maximum residue limits (MRLs) for several fluoroquinolones such as DAN, enrofloxacin (ENR) and ciprofloxacin (CIP) in bovine milk is in the range of 30‐100 μg/kg (Bulletin of the Ministry of Agriculture of the People’s Republic of China, No 235, 2002)
High‐performance liquid chromatography (HPLC)-grade methanol was purchased from Fisher Scientific, Inc. (Pittsburgh, USA)
Summary
Danofloxacin (DAN), one of the most important fluoroquinolones, has been widely used as veterinary medicines for farm animals to prevent and treat various infection diseases. Adverse effects reported by long-term exposure to fluoroquinolones include central nervous system toxicity, cardio toxicity, arthropathy and tendon toxicity (Licata et al, 2012). For these reasons, the employment of fluoroquinolones in animal production is regulated and maximum residue limits (MRLs) have been established by the European Union (Regulations CE No 37/2010). There is an urgent need to develop a simple, effective and accurate analytic method to quantify DAN residues in livestock products
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