Abstract
AbstractThe development and characterization of a novel antibacterial material based on glucose oxidase (GOx) immobilized in a polyvinyl alcohol (PVA) film was proposed. This system acts in the presence of glucose to generate by-products as oxygen species (H2O2, ·O2 −, OH) that are well-known endogenous and exogenous toxic products for microbes in vivo (Miller and Britigan, Clin Microb R10: 1–18, 1997). The PVA/GOx composite material has been extensively characterized by X-ray photoelectron (XPS) spectroscopy, Fourier transform infrared (FTIR), and UV-visible spectroscopy (UV–vis) to verify the preservation of the enzyme structural integrity and of the enzymatic activity in PVA membrane. The antibacterial lysozyme-like activity of PVA/GOx was evaluated by a standard assay on Petri dishes employing Micrococcus lysodeikticus dried cell walls.KeywordsComposite FilmHybrid FilmVinyl AlcoholTriglycine SulfateAntibacterial MaterialThese keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.
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