Abstract
We isolated and characterized microsatellite loci for the coral reef fish Abudefduf saxatilis using Illumina paired-end-sequencing and tested 48 loci for amplification and polymorphism. We screened 29 polymorphic loci in individuals from Reserva de la Biosfera Banco Chinchorro (RBBC), Quintana Roo, Mexico to assess variability. Number of alleles per locus varied from 8 to 20, observed and expected heterozygosities from 0.458 to 1.00 and from 0.820 to 0.931, respectively. Some of the primers amplified individuals of four congeneric species. These markers will constitute invaluable tools for elucidating patterns of connectivity among populations of coral reef fishes, providing important information for the conservation of coral reefs.
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