Abstract
18 novel microsatellite loci were separated by the method of triplex affinity capture for Leptobotia elongate. All these 18 loci were polymorphic in L. elongate and the number of alleles per locus ranged from 5 to 14. The observed and expected heterozygosity were 0.259–0.966 and 0.486–0.918, respectively. Deviation from Hardy–Weinberg equilibrium was indicated at four loci. The significant pairwise linkage disequilibrium was found in one pair of loci. This is the first development of species-specific markers for L. elongate. These microsatellite loci would provide new opportunities for a wide range of population genetic and taxonomic studies of L. elongate.
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